5. UV-Vis Spectroscopy5.1 Introduction
Objective
Review basic analytical chemistry skills, including solution making, transferring and linear diluting. Understand the error involved and error propagation.
Materials:
Methylene Blue C16H18ClN3S, MW 319.85.Also called Swiss blue. One gram dissolves in about 1000 ml of water. Peak absorption at 369 nm
5.2 Create Calibration Curve from standard solutions
Procedures:

Weigh 500mg dye on electric balance
Dissolving all the solid dye in beaker
Transfer all the solution into a 500ml volumetric flask
Fill up the volumetric flask to the tick mark
Prepare standard solutions of 500, 200, 100, 50 and 20 ppm from 1000 ppm stock

solution
Table 5-1 Preparation of standard solutions

PPM

STD

Stock